Figure 4
From: Stromal cells as trend-setters for cells migrating into the lymph node
Stromal cells (SCs) from peripheral LN (pLN) change their expression pattern to that of mesenteric LN (mLN). Neotx-mLNmes and Neotx-pLNmes were removed 8 weeks after transplantation and SCs were sorted into an EGFP+ and EGFP− fraction using a FACSAria IIu. mRNA was isolated and real-time PCR in triplets was performed. All SCs isolated from Neotx-LNmes showed a similar expression of genes compared with the mLN control. The data are normalized to the housekeeping gene β-actin and given from four animals. Significant differences in the unpaired t-test are indicated by *P<0.05; **P<0.01; ***P<0.001.
