Figure 5
From: Stromal cells as trend-setters for cells migrating into the lymph node
Transferred antigen-specific lymphocytes proliferate and induce gut-homing molecules in Neotx from isolated peripheral LN (pLN) stromal cells (SCs). Eight weeks after transplantation, CFSE, 5-(and 6)-Carboxyfluorescein diacetate succinimidyl ester-labeled spleen cells isolated from OT-II mice were adoptively transferred into transplanted (wild-type recipients (Neotx-LNmes) or RORγt recipients (Neotx*-pLNmes)) and non-transplanted control recipients. One day after transfer, mice received a single administration of ovalbumin (Ova), and after 3 days lymph nodes (LNs) were excised and CCR9 and α4β7 integrin upregulation was determined with flow cytometry. OT-II cells were found in all lymph node (LN) in similar percentages (a). Neotx-LNmes induced gut-homing molecules independent of the origin of SCs, which generated the LN, whereas no upregulation was identified in Neotx-LNpop (b). The means and s.e. are given from four to eight independent experiments (significant differences in the unpaired t-test are indicated by *P<0.05; **P<0.01; ***P<0.001).
