Figure 1
Glycoprotein 2 (GP2) expression defines two microfold (M)-cell subsets. (a) A confocal image of a whole-mount immunostaining of the isolated follicle-associated epithelium (FAE) monolayer from an ileal Peyer’s patch of a BALB/c mouse for Tnfaip2 (green) and GP2 (magenta). (b) An enlarged view of the squared area in a. (c) Histograms of fluorescence intensity obtained from the confocal images. The x axis represents fluorescence intensity in arbitrary units on a log10 scale and the y axis is the number of cells found at each fluorescence level (normalized to mode). Red lines represent cells stained with either the anti-Tnfaip2 or GP2 antibody. Gray lines show background fluorescence intensities obtained from control experiments without primary antibodies. (d) Scatter plot of the fluorescence intensities of Tnfaip2 versus GP2, where each dot represents a single cell. The capital N indicates the number of analyzed cells. The proportions of cells within the indicated boxed areas were calculated by FlowJo software as described in Methods. Bars = 200 μm (a) and 50 μm (b).
