Figure 6
RelB activity is essential for microfold (M)-cell differentiation. (a) Western blot analysis of nuclear factor-κB (NF-κB) proteins in the ileal and cecal epithelium with sequential treatment with receptor activator of NF-κB ligand (RANKL). Epithelia were dissected at indicated days of the sequential administration. Nuclear fractions were isolated and the obtained samples were subsequently subjected to western blotting for indicated proteins in figures as described in Methods. Data are representative of two independent experiments. Optical densities were measured from scanned X-ray films and normalized to densities of lamin A/C (right panels). (b) Whole-mount immunohistochemical images of Tnfaip2 and Glycoprotein 2 (GP2) in ileal epithelia of ALY/NscJcl-aly/aly (aly/aly) or ALY/NscJcl-aly/+ (aly/+) mice injected with glutathione S-transferase (GST) or GST-RANKL. Administrations of GST or GST-RANKL were performed as described in Methods. The aly/+ mice were used as controls. The ileal specimen is composed of villi and many crypts. (c) Quantitative PCR analysis of the expression of M-cell markers. Results are normalized to Gapdh expression and are presented relative to the expression in the ileal epithelium from GST-treated mice. Data are representative of four animals per group. Error bars indicate s.d. Bars = 50 μm (a).
