Figure 8
Small numbers of mmicrofold (M) cells in the cecal follicle-associated epithelium (FAE) express nuclear RelB. (a) Histograms of the fluorescence intensity of nuclear RelB in the ileal FAE and the cecal FAE of C57BL6/N mice. Nuclear fluorescence intensities were measured from images of whole-mount immunohistochemistry with an antibody against Spi-B and RelB as described in Methods. Black lines represent cells stained with the anti-RelB antibody and gray lines represent cells stained without the anti-RelB antibody. (b) Scatter plots of the fluorescence intensities of RelB versus Spi-B. Over 8,000 cells were counted from more than 10 whole-mount images of FAE from 3 C57BL/6 mice. The numbers indicated in the scatter plots are the proportions of each boxed area to the whole cells. Data are representative of three independent experiments. (c) Scatter plots summarizing the proportions of RelB-positive cells in total FAE cells (left) or in total M cells (Spi-B-positive cells) (right). ***P<0.001; P-values were calculated by Dunnett’s test.
