Figure 9
Adora2b (A2B adenosine receptor) agonist treatment results in significantly increased localization of p-VASP (phosphorylated vasodilator-stimulated phosphoprotein) (Ser157) to the intestinal epithelial barrier in vivo during DSS (dextran sulfate sodium) colitis. Gender-, age-, and weight-matched C57BL/6 mice were treated with an Adora2b-specific agonist (BAY 60-6583; 1.2–1.25 mg/kg−1 per day) or vehicle for 6 days during DSS as described in Figure 4. Colons were harvested by blunt dissection and formalin fixed. (a) Formalin-fixed colons were deparaffinized and rehydrated. Sections were costained with p-VASP (Ser157) and E-cadherin antibodies as described in Figure 7a and b. Representative images were acquired at original magnification × 20. Bar=50 μm. (b) p-VASP (Ser157) staining of E-cadherin-positive cells was calculated as described in Figure 7c. Results are representative of 1–3 images per mouse with 5–7 mice per group. Statistical analysis was performed using the Student’s t-test. Data are displayed as the mean±s.e.m. ***P<0.0001.
