Figure 5
Restoration of intestinal epithelial barrier integrity in response to treatment-induced remission in mice with colitis and contribution of tumor necrosis factor α (TNFα) in colitis induction and progression. (a) Serum TNFα protein levels in naive Rag−/− H+ mice, mice with active disease, or mice in remission in colitis on days 1, 3, and 11 days after anti-CD4 depletion (postdepletion (p.d.)). (b) Fecal lipocalin-2. (c) Fecal albumin. Pooled data from 5 independent experiments with n=2–11 mice per group are shown. Graphs represent mean values of individual mice. Statistics compare naive controls vs. mice with active disease vs. mice in remission using one-way nalysis of variance with Tukey’s multiple comparison test. *P<0.05; **P<0.01; ***P<0.001. (d,e) Percentage of body weight change in response to (d) therapeutic or (e) preventive anti-TNFα treatment compared with anti-CD4 therapeutic treatment. (f) Colonic histopathology scores of naive H+ Rag−/− mice compared with mice that were treated with anti-TNFα prior colitis induction (prevent.), mice with active disease treated with anti-TNFα (therap., green arrows), or mice with active disease treated with anti-CD4 antibodies (black arrows) at the indicated time points. Data with n=4–6 mice per group are shown.
