Figure 6
Neutralization of tumor necrosis factor α (TNFα) during active colitis does not alter concentrations of other pro-inflammatory mediators. Protein levels for TNFα, interferon-γ (IFNγ), interleukin-17A (IL17A), IL6, IL1α, C-X-C chemokine ligand 9 (Cxcl9), and ganulocyte colony-stimulating factor (G-CSF) in (a) colon homogenates and (b) sera of H+ Rag−/− mice. H+ Rag−/− mice were transferred with colitogenic CD4+ CD45RBhi T cells to induce colitis and either treated with anti-TNFα prior to transfer of colitogenic T cells (preventive treatment), during active disease with anti-TNFα (therapeutic treatment), or with anti-CD4 to induce remission. Mice were analyzed on days 12/13 after transfer (active disease and preventive anti-TNFα), on day 4 after treatment (therapeutic anti-TNFα), and on day 11 after depletion (anti-CD4). Serum and colon homogenate samples were analyzd by bead-based multiplex assay for cytokines. Mean values±s.d. are shown from pooled data of two independent experiments with n=4–6 mice per group. Statistics compare the different groups using one-way analysis of variance with Dunn’s multiple comparison test. *P<0.05; **P<0.01. Prev, preventive; Therap, therapeutic.
