Figure 10
The adoptive transfer of alveolar macrophages (AMΦs) from donor Cd163−/− mice enhances Dermatophagoides pteronyssinus peptidase 1 (Der p1)-induced increases in eosinophilic airway inflammation and mucous cell metaplasia (MCM) in recipient wild-type (WT) mice. (a) WT mice were sensitized by intraperitoneal (i.p.) injection of Der p1 or saline, as a control, on days 1 and 7, followed by the intranasal adoptive transfer of 1 × 105 AMΦs from naïve donor WT or Cd163−/− mice on day 14. All mice received an intratracheal Der p1 (10 μg) challenge on day 17 and end points were analyzed on day 20. (b) Gating strategy for sorting of naive murine alveolar macrophages in bronchoalveolar lavage fluid (BALF). Cellular debris was excluded using a forward light scatter/side scatter plot and doublets were excluded using width parameter on forward light scatter (FSC) and side light scatter (SSC) properties. CD45+ cells that co-expressed CD11c, Siglec-F, and CD64 were identified as AMΦs. A microscopic image of sorted CD45+/CD11c+/Siglec-F+/CD64+ cells shows a cellular population possessing typical characteristics of AMΦs. (c) Total BALF inflammatory cells and inflammatory cell types (n=4–8 mice, *P<0.05, donor Cd163−/− AMΦs vs. donor WT AMΦs). (d) BALF CCL24 levels from Der p1-challenged recipient mice (n=8 mice, P=0.0016, donor Cd163−/− AMΦs vs. donor WT AMΦs, unpaired t test). (c, d) Representative data from one of three independent experiments are shown. Numbers of mice that were included in each experimental condition are shown. The same numbers of mice are shown for both analyses in (c).
