Figure 1
Varying perforin and GrzB expression between blood and rectal CD8+ T-cells and across HIV-infection. (a) Representative flow cytometry plot of intracellular perforin and GrzB expression in unstimulated ex vivo blood and rectal mucosal CD8+ T cells from representative participants: viremic untreated (top) and seronegative (bottom). (b) Difference in intracellular perforin and GrzB expression between blood and rectal CD8+ T cells in a consolidated group of all HIV+ participants compared with seronegative (SN) participants. (c) Difference in intracellular perforin and GrzB expression in rectal and blood CD8+ T-cells across HIV-disease status. (d) Spearman correlation analysis relating the frequency of GrzB+ CD8+ T-cells with the frequency of perforin+ CD8+ T-cells in rectal mucosa and blood. Wide horizontal bars represent medians; narrow whiskers indicate interquartile ranges; asterisks show level of significance as follows: *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.
