Figure 3
From: Norovirus antagonism of B-cell antigen presentation results in impaired control of acute infection
Antiviral antibody responses do not account for B-cell-mediated control of MNV-1 infection. Groups of B6 mice were infected p.o. with either 104 TCID50 units (a) or 107 TCID50 units (b) of MNV-1 (black lines) or MNV-3 (gray lines). At 0, 1, 3, 5, 7, 14, 21, and 28 d.p.i., fecal pellets and serum were collected from each mouse. Virus-specific antibody was detected by standard ELISA using an anti-mouse IgA secondary antibody for fecal lysates, and anti-mouse IgM, IgG, and IgA secondary antibodies for serum samples. The data are reported as the averaged absorbance readings for all mice per condition (n=6 mice over two experiments) subtracted by the absorbance of samples collected at 0 d.p.i. The two virus strains were compared at each time point for statistical purposes. d.p.i., days post infection; ELISA, enzyme-linked immunosorbent assay; p.o., perorally.
