Figure 4
Phagocytosis of H. capsulatum by eosinophils results in interleukin (IL)-4 secretion. (a and b) Flow cytometric analysis of bone marrow-derived eosinophils challenged with 1 or 5 multiplicity of infection (MOI) GFP+ H. capsulatum yeasts for 6 h. Plots representative of one of five independent experiments. (c and d) Measurement of IL-4 protein concentration in culture supernatants of eosinophils infected with different MOI of H. capsulatum yeasts for varying time points as indicated. Values are mean±s.e.m. from at least four independent experiments. *P<0.05, **P<0.01 as compared with control, either time 0 for c or uninfected for d.
