Figure 7

Association of H. capsulatum with eosinophils in vivo. (a) Flow cytometric analysis of H. capsulatum-infected eosinophils in vivo. CCR2−/− mice were infected with 1 × 107 GFP+ H. capsulatum yeasts and GFP+ eosinophils in the lungs were analyzed by flow cytometry 3 days post infection (p.i.). Plot representative of one of six mice from two independent experiments. (b) Microscopic analysis of GFP-Hc-infected eosinophils from lungs of CCR2−/− mice, 3 days after challenge and analyzed with an ImageStreamX flow cytometer.