Figure 3
From: Critical role of CXCL4 in the lung pathogenesis of influenza (H1N1) respiratory infection
Chemokine (C-X-C motif) ligand 4 (CXCL4) knockout results in impaired viral clearance. Lungs were harvested from H1N1 A/PR/8 (2,000 CCID50) (50% cell culture infective dose) infected wild-type (WT) and CXCL4−/− mice, and homogenates were prepared for viral titers and loads. (a) Titers of infectious virus were determined using a standard CCID50 assay at different days postinfection (d.p.i.). (b) Viral loads were determined based on the number of influenza M gene RNA copies detected by real-time reverse transcriptase–PCR at different d.p.i. Error bars represent the s.d. of triplicate samples. *P<0.05 based on Student’s t-test.
