Figure 2
From: Protective role of γδ T cells in cigarette smoke and influenza infection
Decreased protective immunity in influenza-infected, smoke-exposed mice. (a) Enzyme linked immune-spot (ELISpot) quantification of interleukin (IL)-17A using single cells isolated from whole lung in the same group of mice on day 14 following influenza infection (n=4 per group) *P<0.05. (b) Whole lung mRNA expression of Ifn-γ, Irf7, and Ifit-3 in the same groups of mice described in Figure 1. (Air, smoke (Smk), Air/Flu, Smk/Flu, n=5) (c) Representative intracellular cytokine (ICC) staining analyses of lung γδT+, CD4+T, and CD8+T cells gated on total lung CD3+ lymphocytes. (d) Cumulative data IL-17A and interferon (IFN)-γ% ICC in γδ, CD4, and CD8 T-cell subsets isolated from the lungs in the same group of mice (n=5 or 6 per group). (e) Quantification of total IgG, IgA, or (f) anti-hemagglutinin (HA)-specific IgG, or IgA antibody in the lung homogenates samples 14 days after influenza infection. Pooled data obtained from at least three different experiments (Air, n=13: Smk, n=13; Air/Flu, n=19, Smk/Flu, n=18). Significance was measured using the Student’s t-test with Bonferroni correction for multiple comparisons *P<0.05, **P<0.01, ***P<0.001.
