Figure 4
From: Protective role of γδ T cells in cigarette smoke and influenza infection
Increased late stage lung viral load and damage in chronic cigarette smoke. Mice (wild-type (WT) and Il-17a−/− mice) in Air/Flu and smoke (Smk)/Flu groups were euthanized at peak (day 6), mid (day 8), and late (day 10) post sublethal dose (25 TCID50) influenza A infection. (a) Quantification of viral load determined using standard plaque assay *P<0.05, **P<0.01, ***P<0.001 (day 6, n=5 per group; day 8 and day 10, n =10 per group. Determined by two-tailed Student’s t-test. Data are representative of two independent experiments. Dashed line indicates the limit of the viral detection. (b) Representative photograph of lung hemorrhage, white dashed lines indicate the boundaries of lung hemorrhagic area (c) quantification of the hemorrhagic scores (% of surface area), (d) lung weight at 6, 8, and 10-days post influenza A infection in the same group of mice described in (a). *P<0.05 (n=5 to 15 per group) using the Student t test with Bonferroni correction.
