Figure 6
From: Protective role of γδ T cells in cigarette smoke and influenza infection
Increased mortality in Tcrδ−/− mice exposed to smoke and influenza. Wild-type (WT) and Tcrδ−/− mice were exposed to air (Air) or smoke (Smk) for 3 months and were infected with sub lethal H3N2 per protocol (Supplementary Figure S1A). (a) Body weight reduction (percent change) following influenza infection (n=7 to 10 mice per group). Data (mean±s.e.m.) are representative of three independent studies using Pearson’s correlation coefficient. (b) Quantification of viral load in the lung from mice (WT and Tcrδ−/− mice) in Smk/Flu groups. Data are presented as mean±s.e.m. and are representative of two independent experiments. *P<0.05. (c) Representative experiment showing total bronchoalveolar lavage (BAL) cell count and differential (macrophages (Mac), eosinophil (Eos), lymphocytes (Lym), and neutrophils (Neu)) in the same group of mice on day 14 following influenza infection described in (a) (n=4 to 8 mice per group). (d) Representative hematoxylin and eosin (H&E) stain of lung tissue section 14 days after influenza A infection and pathology score (e). Scale bars, 100 μm. **P<0.01, ***P<0.001, ****P<0.0001 (f) Representative intracellular cytokine (ICC) staining analyses of lung CD4+ T, CD8+ T cells gated on total lung CD3+ lymphocytes. (g) Cumulative data interleukin (IL)-17A and interferon (IFN)-γ% ICC in CD4, CD8 T-cell subsets isolated from the lungs in the same group of mice (n=3 to 6 per group). *P<0.05, **P<0.01 using the Student’s t-test with Bonferroni correction for multiple comparisons.
