Figure 1
From: Human ASH1 expression in prostate cancer with neuroendocrine differentiation
Several runs with serial control cDNA dilutions were performed. The slope of the plot Ct vs log of input cDNA was −3.28 for hASH and −3.32 for β-actin (being −3.33 100% efficiency). The two efficiencies were comparable at a satisfactory level (<5%) with a correlation coefficient (R2) >0.98. Thus, the slope of the ΔCt values vs log of input cDNA was <0,1 (not shown).
