Figure 2

hASH1 nuclear protein was expressed in normal neuroendocrine cells of the prostate (a; double immunohistochemical procedure with chromogranin A, blue cytoplasmic and hASH1—brown nuclear), pure neuroendocrine carcinomas primary of the prostate (b) and in tumor areas of conventional adenocarcinomas with neuroendocrine differentiation (c), with partial coexpression with Ki-67 (d; hASH1 in red, Ki-67 in green and coexpression in yellow—bottom left) (a; double immunohistochemical procedure, immunoperoxidase and immunoalkaline phosphatase, × 1000; (b) left: H&E × 200, right: chromogranin A—top—and hASH1—bottom—immunostaining, × 200; (c) chromogranin A—left—and hASH1—right—immunostaining in the same tumor area, immunoperoxidase, × 200 and 400; (d) double immunofluorescence procedure, see text for details).