Figure 3 | Modern Pathology

Figure 3

From: Primary cutaneous CD4-positive small/medium-sized pleomorphic T-cell lymphoma: a clonal T-cell lymphoproliferative disorder with indolent behavior

Figure 3

Molecular genetic studies in primary cutaneous CD4-positive small/medium-sized pleomorphic T-cell lymphoma, case 1. (a) Results of a PCR-based assay using primers for T-cell receptor γ-chain gene, analyzed by capillary electrophoresis. Red arrows indicate clonal peaks. Each color represents fragments amplified by primers corresponding to different exons of the γ-gene: green (exon V9/Vγ2), blue (exons V2 to V8/Vγ1), and black (exon V10/Vγ3). The range corresponding to exon V11/Vγ4 showed no amplification and cannot be seen in this figure. (b) Southern blot analysis of the T-cell receptor genes using probes for Jβ2. Lane 1, DNA extracted from case 1; lane 2, negative control; lane 3, positive control. Red arrows indicate clonal rearrangement bands; black arrows indicate germline bands.

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