Table 1 Antibodies used in immunohistochemical assays

From: Adrenal gland hemorrhage in patients with fatal bacterial infections

Antibody against

Animal source

Dilution

References

Neisseria meningitidis a

Horse

1:500

14

Group A streptococcusb

Rabbit

1:2000

15

Rickettsia rickettsii c

Rabbit

1:500

16

Streptococcus pneumoniae d

Rabbit

1:3000

17

Staphylococcus aureus e

Rabbit

1:500

18

Ehrlichia spp. f

Dog

1:100

19

Bacillus anthracis cell wall

Mouse

1:200

20

Leptospira spp. g

Rabbit

1:400

21

Clostridium spp. h

Rabbit

1:1000

18

Klebsiella pneumoniae i

Rabbit

1:200

 

Legionella pneumophila

Rabbit

1:10 000

22

Yersinia pestis

Rabbit

1:3000

23

Treponema pallidum

Rabbit

1:200

24

  1. aThe N. meningitidis antibody does not react with group A streptococcus (GAS), S. aureus, Listeria monocytogenes, B. anthracis, Francisella tularensis, Haemophilus influenzae, Mycoplasma pneumoniae, Pseudomonas spp., or R. rickettsii.
  2. bThe GAS antibody does not react with S. pneumoniae, group B streptococcus (GBS), S. aureus, B. anthracis, Clostridium spp, L. monocytogenes, H. influenzae, Mycobacterium tuberculosis, N. meningitidis, or R. rickettsii.
  3. cTh Rickettsia spp. antibody will cross-react with various spotted fever group rickettsiae, including R. rickettsii, R. akari, R. parkeri, R. africae, and R. conorii.
  4. dThe S. pneumoniae antibody does not react with GAS, GBS, S. aureus, B. anthracis, F. tularensis, L. pneumophila, Leptospira spp., H. influenzae, K. pneumoniae, M. pneumoniae, M. tuberculosis, N. meningitidis, or Y. pestis.
  5. eThe S. aureus antibody does not react with GAS, GBS, S. pneumoniae, B. anthracis, Clostridium spp., K. pneumoniae, Pseudomonas spp., or N. meningitidis.
  6. fThe Ehrlichia spp. antibody cross-reacts with E. canis, E. chaffeensis and E. ewingii.
  7. gThe Leptospira antibody is a mixture of 16 reference rabbit polyclonal anti-leptospira antisera.
  8. hThe Clostridium antibody reacts with various clostridia, including C. perfringens, C. botulinum, C. sordelii, C. novyii, and C. subterminale.
  9. iThe K. pneumoniae antibody does not react with GAS, GBS, S. pneumoniae, B. anthracis, Clostridium spp., F. tularensis, H. influenzae, L. pneumophila, Leptospira spp., N. meningitidis, M. pneumoniae, or R. rickettsii.
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