Table 1 Primer sequences and conditions for quantitative methylation-specific PCR (qMSP) of the CpG-island promoters of the 3p22 genes
Gene | Sequence (5′–3′) | Size (bp) | Tm (°C) | |
|---|---|---|---|---|
MLH1 | Forward | CGTTAAGTATTTTTTTCGTTTTGCG | 214 | 61 |
C-region | Reverse | TAAATCTCTTCGTCCCTCCCTAAAACG | ||
AB002340 | Forward | GCGGTTCGGTTATTTAGTCG | 146 | 61 |
Reverse | CCACTCAACAACGACGTACAC | |||
ITGA9 | Forward | GTCGTTTTTGTGTTCGTTTTTAGC | 109 | 64 |
Reverse | CCGAAACGAAAACTCTACGCCTAAAC | |||
PLCD1 | Forward | GGGCGTCGGATTTTATACG | 133 | 61 |
Reverse | GAACCGCGAACCCTATCATTAC | |||
DLEC1 | Forward | GTAGTTTGCGTTGGCGTAGC | 103 | 63 |
Reverse | ACGAAAAACGCCGATAAACA | |||
MyoD | Forward | CCAACTCCAAATCCCCTCTCTAT | 163 | 61 |
control | Reverse | TGATTAATTTAGATTGGGTTTAGAGAAGGA |
