Table 2 Antibody information and immunohistochemistry conditions

From: Renal cell carcinoma with novel VCL–ALK fusion: new representative of ALK-associated tumor spectrum

Antibody

Manufacturer, location

Clone

Working dilution

Exposure time/ temperature

Staining system

ALK 11

Morris Lab, St Jude, Memphis, TN, USA

Rabbit polyclonal

1:400

32 min/37 °C

Benchmark XT

ALK/p80

Thermo Fisher Scientific, Fremont, CA, USA

5A4

1:40

15 min/room temperature (RT)

Benchmark XT

Vinculin

Sigma-Aldrich, St Louis, MO, USA

hVIN-1

1:1600

32 min/37 °C

Benchmark XT

Vinculin

Abcam, Cambridge, MA, USA

V284

1:100

32 min/37 °C

Benchmark XT

TFE3

Santa Cruz Biotechnology, Santa Cruz, CA, USA

Goat polyclonal

1:600

Overnight/4 °C

BondMax

CK7

Dako, Carpinteria, CA, USA

CD7-272

1:200

32 min/37 °C

BondMax

CK AE1/AE3

Dako

AE1/AE3

1:50

32 min/37 °C

Benchmark XT

CK Cam 5.2

BD Biosciences, San Jose, CA

Cam 5.2

1:10

15 min/RT

BondMax

p53

Dako

DO-7

1:50

32 min/37 °C

Benchmark XT

BAF-47 (INI1)

BD Biosciences

25

1:200

15 min/RT

BondMax

Ki-67

Dako

MIB1

1:200

32 min/37 °C

Benchmark XT

CD10

Leica Microsystems, Bannockburn, IL, USA

56C6

Ready to use

15 min/RT

BondMax

WT1

Dako

6F-H2

1:25

32 min/37 °C

BondMax

S-100

Dako

Rabbit polyclonal

1:800

32 min/37°C

Benchmark XT

Anti-human melanosome

Dako

HMB-45

1:20

15 min/RT

Benchmark XT

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