Figure 4
Colocalization of THBS4 and cytokeratin, vimentin and α-smooth muscle actin in human diffuse-type gastric adenocarcinomas. Simultaneous fluorescent immunohistochemical detection of THBS4 in red and pan-cytokeratin (KRT), vimentin (VIM) or α-smooth muscle actin (αSMA) in green was performed on 10 μm thin cryosections. Signals were scanned using a confocal laser scanning microscope. Representative pictures of single confocal sections are shown, respectively. Negative controls were obtained by omission of primary antibodies and scanned with identical settings (pinhole, excitation, frame average, etc.) to positive stainings, respectively (data not shown).
