Figure 5
THBS4 mRNA expression in human diffuse-type gastric adenocarcinoma-derived cancer-associated fibroblasts and healthy counterpart-derived normal fibroblasts. Two cell lines of matched diffuse-type gastric adenocarcinoma-derived cancer-associated fibroblasts (CAF-32, CAF-33) and healthy counterpart-derived normal fibroblasts (NF-32, NF-33) were analyzed. The mRNA abundance was examined by means of quantitative real-time PCR. Quantitation was done relative to the transcript of actin, β (ACTB) and expression levels in NF-lines were set to 100%, respectively. Significance in differential expression was assessed using t-test (**P<0.01; ***P<0.001). Error bars represent integrated standard errors of the mean.
