Figure 3
Distribution of the viral and cellular markers E4, L1 and minichromosome maintenance protein 7 (MCM7) in a bowenoid actinic keratosis from a kidney transplant recipient (patient 8, neck). The top pictures (scale bars: 1000 μm) show the scan of the tissue sections using hematoxylin and eosin (H&E) staining; the right-hand panel shows an actinic keratosis with the area of interest boxed in red and the left panel shows a nearby squamous cell carcinoma. The region shown in the lower panels corresponds to the red square highlighted in the H&E image, magnified in the left-hand lower picture. The same section was double stained using antibodies to E4 (green) and MCM7 (red) (middle picture) and a serial section was stained with antibodies to L1 (red) (right picture). The white arrows indicate nuclear L1 staining. All sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (blue) to visualize cell nuclei. Scale bars: 50 μm.
