Figure 2
(a, b) In the liver of itai-itai disease cases, fibrosis was observed in a stellate pattern around the portal region; (a) H&E, × 200, (b) Azan, × 200. (c, d) Iron accumulation was mainly in hepatocytes with a massive pattern in itai-itai disease cases with hemosiderosis; (c) H&E, × 200, (d) Berlin blue, × 200. (e) Inflammation observed in itai-itai disease cases was mainly located in portal regions. The infiltrating cells were primarily lymphocytes; H&E, × 200. (f) Macrovesicular and microvesicular fatty changes were mixed in itai-itai disease cases with steatosis; H&E, × 200. (g) Metallothionein was expressed in the cytoplasm and nuclei of hepatocytes in the liver of itai-itai disease cases; metallothionein immunohistochemistry, × 400. (h) Metallothionein was weakly expressed only in the cytoplasm of hepatocytes in the liver of control cases; metallothionein immunohistochemistry, × 400.
