Table 2 Immunohistochemical study protocol
From: A novel morphological approach to gonads in disorders of sex development
Antibody | Clone | Dilution | Pre-ttt | HIER | Incubation | Revelation | Chromogenic | Staining |
|---|---|---|---|---|---|---|---|---|
Inhibin (α-subunit) | Monoclonal mouse Ab Clone R1 SEROTEC MCA951S | 1/200 | CC1 std | 99 °C | 32 min | HRP Multiplex | DAB | Cytoplasmic |
AMH | Monoclonal mouse Ab Clone 5/6 ACRIT AMO5878SU | 1/20 | Cytoplasmic | |||||
CD117 | Polyclonal rabbit Ab DAKO A4502 | 1/200 | Cytoplasmic granular with membranous enhancement | |||||
OCT4 | Monoclonal mouse Ab ROCHE MRQ-10 | Ready to use | Nuclear+/−cytoplasmic granular |
