Figure 6
From: Distinct patterns of primary and motile cilia in Rathke’s cleft cysts and craniopharyngioma subtypes
ARL13B immunohistochemistry (red) on adamantinomatous craniopharyngioma (a) and papillary craniopharyngioma (b). Adamantinomatous craniopharyngiomas diffusely express primary cilia, in both the palisading basal cells and non-palisading 'apical' cells in 100% of cases. Papillary craniopharyngiomas express primary cilia in basally oriented cells near fibrovascular cores in 100% of cases, but show no expression of primary cilia in more 'apical' cells. Statistical analysis of primary cilia in different lesions (c). Differences in expression between 'apical' and 'basal' cells are statistically significant (****P<0.05). Matched serial sections of an adamantinomatous craniopharyngioma with hematoxylin and eosin (H&E) (d), RNAscope in situ hybridization for SHH (e) or PTCH1 (g) mRNA, or immunohistochemistry for β-catenin (f). Regions of high SHH mRNA expression (e, arrows) show excellent correlation with regions of nuclear/cytoplasmic β-catenin translocation (f, arrows). Statistical analysis of PTCH1 and GLI2 expression in adamantinomatous craniopharyngioma and papillary craniopharyngioma (h). Differences in expression are statistically significant (****P<0.05). Scale bars, 20 μm (a and b) or 50 μm (d–g).
