Figure 3
From: Molecular testing for the clinical diagnosis of fibrolamellar carcinoma
Fibrolamellar carcinoma with PRKACA locus amplification. (a) PRKACA break Apart FISH results are consistent with amplification of the PRKACA locus. The fibrolamellar carcinoma showed typical morphology. There are greater than 10 separate green signals per tumor cell nucleus with fewer intact yellow signals. (b) Quantitative reverse transcription-PCR shows a 2–5-fold increase in PRKACA mRNA compared to four randomly selected fibrolamellar carcinomas (FL-HCC-2-5) and one randomly selected cholangiocarcinoma (CLC).
