Figure 5
From: Estrogen-dependent association of HDAC4 with fear in female mice and women with PTSD
Tone-shock exposure mediated alterations in Hdac4 expression are influenced by estrogen status. (a) Mouse amygdala Hdac4 immunohistochemistry (red=Hdac4; blue=4,6-diamidino-2-phenylindole/Hoechst). (b) Amygdala Hdac4 mRNA expression in ovariectomized mice in the presence (Est) or absence (Ovx) of estrogen 2 h after tone-shock exposure (FC) compared with homecage (HC) controls. Mean Hdac4 foldchange relative to HC-Ovx and normalized to Gapdh foldchange. (c) Amygdala Hdac4 level in naturally cycling female mice in either metestrous (Met) or proestrous (Pro) 2 h after tone-shock exposure (FC) compared with HC controls. Mean Hdac4 foldchange relative to HC-Met and normalized to Gapdh fold change. BLA, basal lateral nucleus of the amygdala; NS, nonsignificant.
