Figure 2: Representative examples of in situ hybridization data of E10.5 (whole mount) and E14.5 (sections) embryos.

See supplementary Information for the list of genes studied and images of their expression patterns. Btg3 messenger RNA is ubiquitously expressed at E10.5, with higher abundance in brain and gut. At E14.5 expression in the brain is restricted to the ventricular zone (red insert) and to a group of cells migrating into the developing cerebellum (black insert). Pcp4 is transcribed in brain, eye (black arrow) and dorsal root ganglia (white arrow) at E10.5. At E14.5 cells transcribing this gene are found in numerous tissues including the cortical plate (red arrow), midbrain, cerebellum, spinal chord, intestine, heart and dorsal root ganglia. Pcbp3 transcripts are restricted to the central and peripheral nervous systems both at E10.5 and E14.5 (white arrow, dorsal root ganglia; red arrow, facial nerve nucleus). Insert shows a group of neuroblasts migrating from the midbrain into the cerebellar anlage. Expression of Pcnt2 at E10.5 is ubiquitous but stronger in brain, eye, limbs, branchial arches and gut. At E14.5 Pcnt2 brain expression is restricted to proliferating cells of the ventricular zone (red insert). At E10.5 Pdxk mRNA is ubiquitous but is more strongly expressed in brain and eye. At E14.5 a group of cells in the inferior colliculus express Pdxk (red insert). Pfkl transcripts are found throughout the E10.5 embryo with higher levels in brain, eye and dorsal root ganglia (white arrow). At E14.5 this transcript is widely expressed but exhibits regional upregulation in the ventricular zone (red arrow), axial and cranial cartilage, thymus, gut and lung.