Figure 3: Expression analysis in the developing heart.
From: Human chromosome 21 gene expression atlas in the mouse
E10.5 whole embryos and E14.5 sections were probed for Adarb1, Atp50, C21orf11, C21orf18, Col6a1, Col18a1, Cldn8, Kcnj15, Kcne2, Pfkl, Pwp2h and Sh3bgr genes. a, The region portrayed from the whole-mount embryos is schematically represented in the red box of the top left panel. h, heart; ba, branchial arch; fl, forelimb. At E10.5, C21orf18 transcripts are present in the aortic sac (red arrow) and the bulbus cordis (black arrowhead). Kcne2 is expressed in atria and ventricles. mRNA for C21orf11 and Pwp2h is restricted to the atria (black arrows), and Cldn8 transcripts are restricted to the primitive ventricle (black arrow). b, Sh3bgr and Atp50 transcripts are detected throughout the heart. Adarb1 mRNA is present in the aortic sac (red arrow) at E10.5, and is restricted to the mitral valve (black arrowhead), aortic valve (red arrow) and the endothelium of the aortic trunk at E14.5. c, At E14.5 Pfkl is expressed throughout the heart. Kcnj15 transcripts are found in the aortic and mitral valve (red arrow and black arrowhead, respectively). Col6a1 is strongly expressed in the mitral valve (black arrowhead) and along the pericardium, whereas Col18a1 expression is seen in the vessels of the heart.
