Figure 11: Genes on chicken microchromosomes possess higher synonymous substitution rates.

We split the chromosomes into macrochromosomes (GGA1–5, grey circles), intermediate chromosomes (GGA6–10, green triangles) and microchromosomes (GGA11–32, red circles), and calculated their KS values using Codeml. The cumulative distribution of genes in each chromosome size category is plotted versus the KS values. Because large KS values are methodologically suspect, we discarded KS values >10.