Nature 444, 481–485 (2006); doi: 10.1038/nature05142 (this issue)

At the request of the Editors at Nature, we wish to clarify some questions that have arisen since the advance online publication (AOP) of our Letter on 23 August 2006. In our Letter, we showed that human embryonic stem-cell lines can be generated from a single cell after its removal from an 8–10-cell embryo. To minimize the number of embryos used, we removed multiple cells from each embryo, and none of the biopsied embryos were allowed to develop in culture.

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