Figure 1: HA and virus binding to human and avian receptors.
From: Receptor binding by a ferret-transmissible H5 avian influenza virus
a, MST data for the binding of α2,3-linked sialyl lactosamine (α2,3-SLN) and α2,6-linked sialyl lactosamine (α2,6-SLN) to HAs. Data are plotted as normalized signal change as a function of receptor sugar concentration divided by 3. For a full explanation of the normalization of the sugar concentration see Supplementary Information. b, BLI data for the binding of viruses to α2,3-SLN and α2,6-SLN. 30-kDa polymers containing 20% mol sugar and 5% mol biotin linked to a polyacrylamide backbone were immobilized to different levels on streptavidin-coated biosensors. Data are plotted as fractional saturation of the sensor surface as a function of relative sugar loading (RSL) for a fixed virus concentration of 100 pM. c, Comparison of experimental and simulated BLI data. The symbols represent the BLI data for the wild-type H5 virus. The solid lines are binding curves simulated using the HA monomer dissociation constants derived from MST experiments and the multiplicity coefficient (see Supplementary Information).
