Extended Data Figure 6: Spontaneous defects, checkpoint indices, damage foci and clonogenic survival of HELQ-deficient cells.
From: HELQ promotes RAD51 paralogue-dependent repair to avert germ cell loss and tumorigenesis
a, Primary Helq mutant and control cell lines were grown in physiological O2 for the indicated number of days and passaged regularly to generate a cumulative population doubling (CPD) curve. Means ± s.d. of triplicate replicas are shown. b, c, Helq mutant and control cells grown on coverslips were formaldehyde fixed and DAPI stained to determine levels of spontaneous micronuclei formation: percentage of 100 cells exhibiting 1 or more micronucleus (b); representative images (c), micronuclei (arrows). d, e, DNA combing used to calculate replication fork rates of primary cells grown under atmospheric (d) or physiological (e) O2. Cells in d were treated with or without 2.5 μM CPT for 15 min during labelling. f, Examples of origin containing IdU (green)- and CldU (red)-labelled fibres. g, Right versus left replication tract lengths to determine fork asymmetry (defined as tracts falling outside the interquartile lines).
