Extended Data Figure 7: Checkpoint and double-strand break repair function.
From: HELQ promotes RAD51 paralogue-dependent repair to avert germ cell loss and tumorigenesis
a, b, Immortalized HelqΔC/ΔC and Helq+/+ cells treated with or without 500 ng ml−1 MMC (a) or 50 nM CPT (b) for 20 h and probed for the indicated checkpoint indices. c, Primary wild-type (WT), HELQ-deficient (HQ) and FANCD2-deficient (D2) cells were left untreated (−), or exposed to 5 Gy irradiation and collected 30 min later, or 3 μM APH for 16 h and harvested 10 min later, and lysates were probed for the indicated checkpoint indices. d, Phospho-Ser 345 CHK1 levels in U2OS cells subjected to 1 µM MMC for 24 h. e, Immortalized mouse cells were treated with 1 µM MMC for 24 h, allowed to recover for the indicated times (in hours), and stained for γH2AX. f, Quantification of percentage of positive cells from e. g, RPA32 and RAD51 foci formation in U2OS cells ± 1 μM MMC for 24 h subjected to control and HELQ siRNA.
