Extended Data Figure 8: Homologous recombination dynamics and PARP inhibitor sensitivity.
From: HELQ promotes RAD51 paralogue-dependent repair to avert germ cell loss and tumorigenesis
a, U2OS cells treated with or without 100 ng ml−1 MMC for 24 h, allowed to recover for the indicated times (in hours), fractionated and probed for RAD51. H4 and α-tubulin are shown as controls for chromatin fractionation. b, DR–GFP reporter cells treated with the indicated siRNAs were probed for BRCA2, HELQ and RAD51; transcription factor IIH (TFIIH) is shown as a loading control. c, siRNA-treated U2OS cells were plated for clonogenic survival and treated with the indicated reagents.
