Extended Data Figure 7: FluBI B cells are not infected with A/Puerto Rico/8/1934 virus in vivo.
From: Antigen-specific B-cell receptor sensitizes B cells to infection by influenza virus
C57BL/6 mice were administered 5 × 106 MHCII–GFP+ FluBI B cells 2 h before intranasal infection with 2 × 105 p.f.u. per mouse of either A/WSN/33 (WSN) or A/Puerto Rico/8/1934 (PR8). Mice were euthanized 3 days post-infection, and lung resident cells were stained with anti-CD19 and TAMRA-conjugated VHH68 (anti-HA) or TAMRA-conjugated VHH52/54 (anti-NP). a, Representative plots gated on CD19+ cells. b, Quantification of flu-antigen positive cells as shown in a. n = 3. Error bars are s.d. p = 0.06 using two-sided t-test.
