Extended Data Figure 10: Flu-specific VHHs can stain infected FluBI B cells.
From: Antigen-specific B-cell receptor sensitizes B cells to infection by influenza virus
B cells from OBI or FluBI mice were cultured for 24 h in RPMI containing anti-CD40 (1 μg ml−1) before exposure to A/WSN/33. OBI B cells, FluBI B cells and MDCK cells were incubated with A/WSN/33 at an MOI of 1.0 for 30 min on ice, washed once with PBS, and transferred to 37 °C in RPMI (0.2% BSA). At 5 h post infection, cells were washed, permeabilized, fixed and stained using TAMRA-conjugated flu-specific VHHs (1 μg in 50 μl). Infected MDCK cells were analysed in parallel as a positive control. Cells were analysed by cytofluorometry using a BD Fortessa.
