Extended Data Figure 3: Sequence of the VDJ and VJ segments of the FluBI antibody.
From: Antigen-specific B-cell receptor sensitizes B cells to infection by influenza virus
Genomic DNA was prepared from tails of FluBI mice. The heavy and light chain rearrangements were first identified by amplifying and sequencing of the segments with degenerate primers: for heavy chain: forward 5′-ARGCCTGGGRCTTCAGTGAAG-3′ and reverse 5′-AGGCTCTGAGATCCCTAGACAG-3′; for light chain: forward 5′-GGCTGCAGSTTCAGTGGCAGTGGRTCWGGRAC-3′ and reverse 5′-ATGCGACGTCAACTGATAATGAGCCCTCTCC-3′. Then the full sequences of the rearranged heavy and light chain segments were obtained using specific primers: forward 5′-TTACTGAGCACACAGGACCTC-3′ and reverse 5′-AGGCTCTGAGATCCCTAGACAG-3′; for light chain: forward 5′-CAGCCCATATTCTCCCATGT-3′ and reverse 5′-ATGCGACGTCAACTGATAATGAGCCCTCTCC-3′. Amplified products were agarose gel-purified and sequenced. Sequences were aligned to the NCBI mouse V, D and J genes using IgBlast. Sequences were deposited in GenBank (accession numbers KF419287 and KF419288).
