Extended Data Figure 7: Gating strategy and isotype controls for iNOS staining of mouse lung.
From: Mycobacteria manipulate macrophage recruitment through coordinated use of membrane lipids
a, Representative gating strategy for isolation of inflammatory monocytes. A dump channel containing anti-CD4, CD8 and CD11c was plotted against a channel exhibiting autofluorescence and also containing anti-Ly6G. Using these markers, T cell, dendritic cell, alveolar macrophage, and neutrophil cell populations were excluded from the double-negative gate. Inflammatory monocytes were identified within the double-negative population by their co-expression of Ly6C and CD11b. These cells were then evaluated for intracellular iNOS expression. a, N = 4 per group (Fig. 3a, b) or b, with isotype control antibodies, N = 4 per group.
