Extended Data Figure 2: ΔmmpL7 bacteria are attenuated in zebrafish larvae.
From: Mycobacteria manipulate macrophage recruitment through coordinated use of membrane lipids
a, Kaplan–Meier graph showing daily survival of larvae infected via caudal vein injection with medium (mock), 29 wild-type or 70 ΔmmpL7 M. marinum. N = 25 (mock), 31 (wild-type), or 29 (ΔmmpL7) larvae per group. Mean time to death (days): mock (11), wild type (7.6) and ΔmmpL7 (11.2). Survival was compared by log-rank test: wild type versus mock and wild type versus ΔmmpL7, P < 0.0001; mock versus ΔmmpL7, P = 0.5601. b, c, Larvae were infected via caudal vein injection 1 dpf with 550 wild-type, 650 ΔmmpL7, or 700Δerp, fluorescent M. marinum. b, Infection burdens were measured by Fluorescent Pixel Count (FPC; mean ± s.e.m.). c, Representative images at 7 dpi. N = 29 (wild-type and ΔmmpL7) or 30 (Δerp) larvae per group. Scale bar, 500 μm. At 3, 5 and 7 dpi, log10 FPC was compared by ANOVA, with Dunnett’s post-test. ***P < 0.001. d, e, Representative images from wild-type (d) and ΔmmpL7 (e) M. marinum HBV infections quantified in Fig. 1d. N = 18 (wild-type) or 16 (ΔmmpL7) larvae per group. HBVs are outlined with a dashed white line. Scale bar, 100 µm.
