Extended Data Figure 3: Knockdown of MyD88 results in a late, dose-dependent hypersusceptibility to M. marinum systemic infection.
From: Mycobacteria manipulate macrophage recruitment through coordinated use of membrane lipids
a, RT–PCR for actin (top) and myd88 (bottom), demonstrating that that the majority of myd88 transcripts at 7 dpf are abnormal in MyD88 morphants. Lanes marked ‘b’ and ‘c’ correspond to morphants from the same experiments depicted in panels b and c, respectively. The abnormal larger transcript (indicated by an asterisk) results from the inclusion of intron 2 in the final transcript, incorporating a premature stop codon that truncates the protein before the TIR (Toll/interleukin receptor) domain. b, c, Caudal vein infection of MyD88 morphants with 141 (b) or 325 (c) c.f.u. M. marinum/larva. Bacterial burden was assessed by FPC, values plotted represent the mean ± s.e.m. Time points were compared by one-way ANOVA and Bonferroni’s post-tests. *** P < 0.001. d, Representative images of larvae at 5 dpi from experiment in c, N = 30 control, 15 MyD88 morphant. Scale bar, 500 μm.
