Extended Data Figure 10: Analysis of PKS genes in the misakinolide chemotype T. swinhoei W1.
From: An environmental bacterial taxon with a large and distinct metabolic repertoire
a, Light micrograph of the enriched ‘Entotheonella’ fraction (n = 3). b, Structure of misakinolide A. c, Phylogram of PKS amplicons generated from the total sponge DNA (blue labels) and the ‘Entotheonella’ fraction (red labels). Black labels belong to known PKS sequences that were retrieved from GenBank and belong to pikromycin (PikAIII), erythromycin (EryKS4), pimaricin (PimS3), myxothiazol (MtaD), barbamide (BarE), nodularin (NdaC), difficidin (DifKS14), onnamide (OnnKS11), bacillaene (BaeKS11) biosynthesis or to synthases putatively involved in production of methyl-branched lipids (SupA). KS numbers refer to the position of the KS in the PKS; that is, sequences were aligned with MUSCLE, and trees were inferred by the neighbour-joining method with 500 replicates, using the Jukes–Cantor correction model and the fatty acid synthase component FabH from E. coli BL21(DE3) as the outgroup. Bootstrap values larger than 50% are shown at the nodes.
