Figure 2: Single-cell analytic studies.
From: An environmental bacterial taxon with a large and distinct metabolic repertoire
a, Differential interference contrast micrograph of the filamentous fraction after differential centrifugation (n = 3). b, Fluorescence micrograph of filamentous bacteria without (top) and with (bottom) ultraviolet excitation (n = 3). c, Scanning electron micrograph of a single filamentous bacterium (n = 3). d, Nested PCR of natural product gene clusters from whole-genome amplification samples of wells sorted with single filaments (n = 48). Wells showing positive amplification for ‘Entotheonella’ sp. 16S rRNA gene, onnamide (onn) and polytheonamide (poy) gene clusters (n = 3) were used for the identification of the other enzyme clusters (n = 1). Each lane represents a single well defined by the well identifier above the top row. cth, cyclotheonamide; ker, keramamide; kon, konbamide; naz, nazumamide. PC, metagenomic DNA from filamentous fraction; pts, unknown proteusin.
