Extended Data Figure 4: Natural product biosynthetic gene loci from ‘Entotheonella factor TSY1’ and ‘Entotheonella factor TSY2’.
From: An environmental bacterial taxon with a large and distinct metabolic repertoire
a–g, Natural product biosynthetic gene loci from ‘Entotheonella factor TSY1’ are shown in a–d, and those from ‘Entotheonella factor TSY2’ are shown in e–g. Biosynthetic gene clusters are numbered according to Supplementary Table 4 and open reading frames (ORFs) are colour-coded based on predicted function. Clusters associated with known compounds are indicated by name. PKS catalytic domains (spheres) are: ACP, acyl carrier protein; AT, acyltransferase; DH, dehydratase; EH, enoyl-CoA hydratase; ER, enoyl reductase; GN, GCN5-like N-acetyltransferase; KR, ketoreductase; KS, ketosynthase; MT, methyltransferase; PS, pyran synthase; TE, thioesterase. NRPS catalytic domains (squares) are: A, adenylation; C, condensation; E, epimerase; M, methyltransferase; NT, aminotransferase; T, thiolation; TE, thioesterase. Amino-acid residues based on adenylation domain specificity (black) or structure-based (red) predictions are indicated below the corresponding adenylation domain. Int, Integrase. Non-proteinogenic amino acids are: mLeu, methyl-leucine; EtGly, ethylglycine (2-aminobutyric acid); Dap, 2,3-diaminopropionate. The clusters are grouped as: non-ribosomal peptide, modular polyketide, and hybrid NRPS/PKS biosynthetic loci associated with TSY1 (a); proteusin gene clusters from TSY1 (b); type III PKS locus from TSY1 (c); ectoine locus from TSY1 (d); NRPS and PKS loci from TSY2 (e); type III PKS locus from TSY2 (f); putative proteusin pathway from TSY2 (g). Several ORFs could not be closed owing to assembly complexity, thus open ORFs are designated with three dashes.
