Extended Data Figure 6: Mass-spectral molecular-network analysis of extracts from enriched ‘Entotheonella’ cell fractions and T. swinhoei.
From: An environmental bacterial taxon with a large and distinct metabolic repertoire
Network analysis was performed as described previously25. Nodes represent consensus spectra and interconnecting edges indicate significant pairwise aligned spectra25. The width of an edge and the distance between two nodes correspond with the relatedness of the two consensus spectra. Related compounds cluster together to form structural families. Nodes were false-colour-coded according to the extract they were derived from: metabolites detected in both the enriched ‘Entotheonella’ fraction and the whole sponge extract are in blue, and metabolites only present in the whole sponge extract are in green. Nodes representing the polytheonamides, orbiculamide A, onnamide A and aurantosides were detected in both the whole sponge and the enriched ‘Entotheonella’ cell pellet. Theopederin D, nazumamide, and several onnamide and aurantoside congeners, as well as the keramamides, were only detected in the whole sponge extract (however, note that keramamides were detected in the ‘Entotheonella’ sample using a more sensitive mass spectrometry method; see Supplementary Table 6). The keramamides are clustering with orbiculamide A, thus suggesting the same biosynthetic origin. In addition, several structural families were detected to be derived from the ‘Entotheonella’ enriched fraction that could not be linked to any metabolites known from T. swinhoei. This observation suggests the presence of as yet unidentified metabolites produced by ‘Entotheonella’.
