Extended Data Figure 10: Effect of anti-miR-25 on accumulation of SUMOylated SERCA2a.
From: Inhibition of miR-25 improves cardiac contractility in the failing heart
Immunoblots of lysates from heart tissue at termination of the in vivo study shown in Fig. 4 (5.5 months after TAC, corresponding to 3.5 months after injection of anti-miR-25 or control (scrambled sequence) anti-miRNA). Lysates were immunoprecipitated with anti-SUMO1 followed by western blotting with anti-SERCA2a, as described previously21. Experiments were replicated three times. Note the reduction in SUMOylated SERCA2a upon TAC in the control anti-miRNA treated hearts, but restoration of expression after specific anti-miR-25 injection. Total levels of SUMO1 and actin are shown.
